4,4'-Dimethoxytrityl chloride—also known as dimethoxytrityl chloride or dimethoxy trityl chloride—is a vital organic compound widely used in the synthesis of oligonucleotides and other DNA/RNA-related applications. With its distinct protective capabilities, DMT-Cl plays a crucial role in modern molecular biology and pharmaceutical industries.

What is 4,4'-Dimethoxytrityl Chloride?

4,4'-Dimethoxytrityl chloride (CAS Number: 40615-36-9) is an organic chemical compound featuring a trityl (triphenylmethyl) core substituted with two methoxy groups in the 4-positions of the phenyl rings. Its chemical formula is C22H21ClO2.

This compound appears as a white to off-white powder and is commonly abbreviated as DMT-Cl. It is highly reactive due to the presence of a chloride functional group, which allows it to easily form ethers when reacting with alcohols. This reactivity makes it a perfect protecting agent for hydroxyl groups during nucleic acid synthesis.

Key Features and Properties

1. Chemical Name: 4,4'-Dimethoxytrityl chloride
2. Synonyms: Dimethoxytrityl chloride, Dimethoxy trityl chloride, DMT-Cl
3. CAS Number: 40615-36-9
4. Molecular Weight: 352.85 g/mol
5. Appearance: White to off-white crystalline powder
6. Solubility: Soluble in organic solvents like dichloromethane, acetonitrile, and chloroform
7. Storage: Store in a cool, dry place, away from moisture and light

Applications of Dimethoxytrityl Chloride

1. Oligonucleotide Synthesis : The most significant application of DMT-Cl is in the solid-phase synthesis of oligonucleotides, such as DNA and RNA chains. DMT-Cl is used to protect the 5’-hydroxyl group of nucleosides during chain elongation. Once a nucleotide is added, the DMT group can be selectively removed with a mild acid, allowing the next nucleotide to bind.This reversible protection is vital for the accurate assembly of custom DNA sequences, making DMT-Cl a cornerstone reagent in genetic research, diagnostics, and therapeutic development.

2. Peptide and Protein Chemistry : In addition to DNA synthesis, DMT-Cl is also used in peptide synthesis as a protecting group for hydroxyl-containing amino acids like serine and threonine. Its selective reactivity ensures the correct sequence and structure during multi-step synthesis procedures.

3. Pharmaceutical Intermediates : DMT-Cl is used in pharmaceutical research and development as a key intermediate. Its ability to create protected forms of various molecules aids in the design of prodrugs and other bioactive compounds.

Benefits of Using 4,4'-Dimethoxytrityl Chloride

1. Selective Reactivity: DMT-Cl selectively reacts with alcohol groups, leaving other functional groups untouched.
2. Reversible Protection: The DMT group is easily removed under mild acidic conditions, which is ideal for stepwise synthesis.
3. High Purity: When sourced from reliable suppliers, DMT-Cl is available in high purity grades suitable for research and commercial applications.
4. Improved Yield: Using DMT-Cl in synthesis protocols often leads to improved product yields and greater overall efficiency.

Handling and Storage

When working with dimethoxytrityl chloride, it is essential to follow proper safety procedures:

1. Wear appropriate PPE: gloves, goggles, and lab coat
2. Use in a fume hood to avoid inhalation
3. Store in an airtight container under dry, cool conditions
4. Keep away from sources of moisture, as it is moisture-sensitive

Why Source DMT-Cl from PharmaNanoAI?

At PharmaNanoAI, we offer high-quality 4,4'-Dimethoxytrityl chloride that meets the stringent demands of the biotech and pharmaceutical industries. Our product is:

1. Laboratory tested for purity and identity
2. Securely packaged to maintain stability during shipping
3. Available in multiple sizes to suit research and industrial-scale needs

Visit our product page to learn more or place an order.

Conclusion

4,4'-Dimethoxytrityl chloride (DMT-Cl) is a powerful chemical reagent that plays a fundamental role in the synthesis of nucleotides and peptides. Whether you're conducting cutting-edge genetic research or developing the next breakthrough drug, DMT-Cl is an indispensable part of your toolkit. With high demand in laboratories worldwide, it’s essential to choose a trusted supplier like PharmaNanoAI for the highest quality and reliability.