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2025-08-14 03:03:01 - Translate -

Introduction, Workflow, and Applications of CRISPR Screening and Sequencing
In competition with bacteriophages, bacteria and archaea have evolved unique ways of defending, which include the CRISPR/Cas systems, the immune systems of bacteria and archaea to resist the invasion of foreign DNAs or RNAs, recognize the foreign invading nucleic acids, and cleave them for immune defense. At present, CRISPR/Cas systems have become efficient and convenient research tools to be...

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kikogarcia bir ses eklendi Health
2025-06-11 08:25:57 - Translate -

Introduction, Workflow, and Applications of CRISPR Screening and Sequencing
In competition with bacteriophages, bacteria and archaea have evolved unique ways of defending, which include the CRISPR/Cas systems, the immune systems of bacteria and archaea to resist the invasion of foreign DNAs or RNAs, recognize the foreign invading nucleic acids, and cleave them for immune defense. At present, CRISPR/Cas systems have become efficient and convenient research tools to be...

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2025-04-09 02:48:50 - Translate -

How to Analyze ChIP-Seq Data: From Data Preprocessing to Downstream Analysis
DNA-protein interactions are widely used to elucidate potential mechanisms of cell physiology. The development of chromatin immunoprecipitation (ChIP) assays has made it possible to study such mechanisms. With further developments, deep sequencing technologies (ChIP-Seq) have emerged, which offer advantages in terms of specificity and sensitivity. In this article, we will provide a detailed...

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2025-04-09 02:46:31 - Translate -

Low-Pass Whole Genome Sequencing: Technology, Applications, and Market Insights
What is Low-Pass Whole Genome Sequencing Low-pass whole-genome sequencing (LP-WGS) is a method involving high-throughput sequencing of entire genomes at relatively low sequencing depths. Typically, LP-WGS operates at a depth of 0.5-1× coverage, meaning each base pair is sequenced, on average, between 0.5 to 1 time. The approach compensates for these sparse data points by...

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2025-02-05 05:55:02 - Translate -

The Principles and Workflow of SNP Microarray
What Is SNP Microarray SNP microarray, a specialized type of microarray technology, is geared towards the detection of Single Nucleotide Polymorphisms (SNPs), characterizing variations at a single base pair in the genome. Emerged in recent years, SNP microarray technology represents a high-throughput, large-scale genetic testing platform. The working principle largely entails the...

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2025-02-05 05:47:49 - Translate -

Overview of Nanopore Sequencing Technology
Abstract Genes encode the entire hereditary information of an organism, and sequencing is the process of converting DNA chemical signals into digitally computable signals, making sequencing indispensable in genomic research. The advent of Sanger sequencing (first-generation sequencing) propelled the realization of the Human Genome Project, marking significant strides in genomic...

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2024-12-23 05:20:27 - Translate -

Detecting Fusion Transcripts by RNA Sequencing in Screening Tumors
What is fusion gene? What is Chimeric RNAs? A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually due to chromosomal translocations or deletions. These chimeric genes can form abnormal transcripts or proteins in subsequent biological processes, which can lead to or promote the development of tumors. For example, in chronic granulocytic leukemia,...

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2024-12-23 05:16:42 - Translate -

Overview of Sequencing Methods for RNA m5C Profiling
Introduction to m5C in RNA m5C is one of the most common and widespread RNA methylation modifications, found primarily in variable arms, anti-codon loops, and regions where rRNA binds tRNA to perform translation. Also dynamically reversible is RNA m5C modification. With S-adenosine methionine (SAM) as the donor, methyltransferase adds a methyl group to cytosine C, forming m5C. m5C can affect...

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2024-10-15 02:51:30 - Translate -

Exploring Nitrogen Cycle Patterns and Mechanisms through Metagenomic Sequencing
The nitrogen cycle stands as one of Earth's paramount biogeochemical cycles, with microorganisms playing an indispensable role in its intricate web. Harnessing the power of metagenomic technologies, we unlock a comprehensive view of both the sheer quantity and the diverse array of functional microbial taxa interwoven within the nitrogen cycle. Consequently, we gain profound insights into...

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2024-10-15 02:42:43 - Translate -

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